Modeling the rod outer segment birefringence change correlated with metarhodopsin II formation
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چکیده
منابع مشابه
Initiation of Rod Outer Segment Disc Formation Requires RDS
Rod outer segment (OS) morphogenesis involves assembly of flattened discs circumscribed by a hairpin-like rim, however, the role of the rim and rim proteins such as retinal degeneration slow (RDS) and its homologue rod OS membrane protein-1 (ROM-1) in this process remains unclear. Here we show that without RDS, no disc/OS formation occurs, while without rhodopsin, small OS structures form conta...
متن کاملTemperature and pH dependence of the metarhodopsin I-metarhodopsin II equilibrium and the binding of metarhodopsin II to G protein in rod disk membranes
The equilibria between metarhodopsins I and II (MI and MII) and the binding of MII to retinal G protein (G) were investigated, using the dual wavelength absorbance response of rod disk membrane (RDM) suspensions to a series of small bleaches, together with a nonlinear least-squares fitting procedure that decouples the two reactions. This method has been subjected to a variety of theoretical and...
متن کاملSialoglycoproteins of the frog rod outer segment plasma membrane.
Photoreceptors can segregate inner segment proteins from outer segment proteins. It is uncertain whether such a sorting process occurs during disc morphogenesis in rods, thereby resulting in an outer segment plasma membrane which differs compositionally from the discs. In this study, the plasma membranes of intact, purified frog rod outer segments (ROS) have been labeled with a peanut lectin (P...
متن کاملMeasurements on the pH dependence of metarhodopsin reactions in sonicated rod outer segments.
The kinetics of rhodopsin photolysis in sonicated suspensions of rod outer segments (R O S), unsonicated ROS suspensions and rhodopsin detergent solutions were compared. No significant kinetic differences were detected; however, the energy of activation for the metarhodopsin I to metarhodopsin II reaction was somewhat higher in sonicated ROS suspensions than in the un sonicated suspensions. W ...
متن کاملRhodopsin in the rod outer segment plasma membrane
Isolated frog retinas were incubated in vitro with a 4-h pulse of [3H]leucine, then chased for 32 h with a nonradioactive amino acid mixture. At the end of the incubation, light and electron microscope autoradiograms were prepared from some of the retinas. The autoradiograms revealed: (a) intense radioactivity in the basal disks of the rod outer segments, (b) diffuse label evenly distributed th...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1982
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(82)84554-2